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Steroid mix on Luna Omega 3 µm C18 150x4.6mm (Batch 3) using Agilent 1260 UHPLC System

Application Detail (App ID: 26509)

Part No:00F-4784-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in Water
Elution B:0.1% Formic Acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 1.5 80 20
3 16.5 55 45
4 19.5 55 45
5 22.5 80 20
6 25 80 20

Flow Rate:2 mL/min
Col. Temp: 30 °C
Detection: LC/UV (DAD, PDA) @ 254.0000000000 nm (nanometers) (0°C)

Analyte Details

Analyte Detail not available

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Hydrocortisone
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Dexamethasone
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3
11-alpha-hydroxyprogesterone
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4
21-hydroxyprogesterone
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5
17-alpha-hydroxyprogesterone
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6
Progesterone
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