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Spinraza on biozen Oligo : Serum load Optimization on micro-Clarity-OTX

Sample Preparation Details

Part Number :8M-S103-4GA
Description :Clarity OTX microelution 2mg/well
Condition 1 :2x 200µl of methanol
Sample Pretreatment :Prep sample right before adding to the well plate to avoid oligo loss to tube, if possible.
Load :rep sample/Bind: in a 300 µl glass vial Lysis buffer + Oligo/Serum in a 1:1 Ratio. Up to 10 µL of Serum . Vortex and transfer to well.
Wash 1 :Wash/Bind: Add 100 µl of equilibration buffer to glass vial where sample was previously stored, transfer to well, use 2.5Hg to wash/bind.
Wash 2 : Equilibration buffer 100µl x 2
Wash 3 : Wash buffer 100µl x 2
Elution 1 : 30 µl of elution buffer x2 for a total of 60µl, elute in a glass coated receiver plate. Inject as soon as sample is eluted
Evaporate: :Evaporate to dryness
Condition 2x 200µl of methanol
Equilibrate Equilibrate: 2x 200 µl of equilibration buffer
Load rep sample/Bind: in a 300 µl glass vial Lysis buffer + Oligo/Serum in a 1:1 Ratio. Up to 10 µL of Serum . Vortex and transfer to well.
Wash Wash/Bind: Add 100 µl of equilibration buffer to glass vial where sample was previously stored, transfer to well, use 2.5Hg to wash/bind.
Wash Equilibration buffer 100µl x 2
Wash Wash buffer 100µl x 2
Elute 30 µl of elution buffer x2 for a total of 60µl, elute in a glass coated receiver plate. Inject as soon as sample is eluted
Notes :1.-Condition: 2x 200µl of methanol 2.-Equilibrate: 2x 200 µl of equilibration buffer 3.-Prep sample/Bind: in a 300 µl glass vial Lysis buffer + Oligo/Serum in a 1:1 Ratio. Up to 10 µL of Serum . Vortex and transfer to well. 4.-Wash/Bind: Add 100 µl of equilibration buffer to glass vial where sample was previously stored, transfer to well, use 2.5Hg to wash/bind. 5.-Wash1: Equilibration buffer 100µl x 2 6.-Wash2: Wash buffer 100µl x 2 7.-Elute: 30 µl of elution buffer x2 for a total of 60µl, elute in a glass coated receiver plate. Inject as soon as sample is eluted

Application Detail (App ID: 27195)

Part No:00B-4790-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:Water + 15 mM N,N-diisopropylethylamine + 35 mM hexafluoroisopropanol
Elution B:90/10 methanol /water + 15 mM N,N-diisopropylethylamine + 35 mM hexafluoroisopropanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 5 60 40
3 5.5 10 90
4 7 10 90
5 7.5 80 20
6 10.5 80 20

Flow Rate:0.25 μL/min
Col. Temp: 70 °C
Detection: LC/MS/MS @ (400°C)
Note:Clarity OTX 2mg micro elution plate

Analyte Details

Analyte Detail not available

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