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Ph. Eur. Azithromycin - Related Substances Standard Solution (6 replicates) on Durashell 5 µm C18(L) 250 x 4.6 mm using Waters® Arc HPLC system

Application Detail (App ID: 27273)

Part No:DC952505-L
Elution Type:Gradient
Elution A:1.80 g/L solution of anhydrous disodium hydrogen phosphate adjusted to pH 8.9
Elution B:Acetonitrile:Methanol = 75:25
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50
2 25 45 55
3 30 40 60
4 80 25 75
5 81 50 50
6 93 50 50

Flow Rate:1 mL/min
Col. Temp: 60 °C
Detection: LC/UV (DAD, PDA) @ 210.0000000000 nm (nanometers) (0°C)
Note:Diluent buffer: 1.73 g/L solution of ammonium dihydrogen pH adjusted to 10.0 with ammonia
Diluent: Diluent buffer: Acetonitrile: methanol = 35:30:35 (v/v/v)

Analyte Details

Analyte Detail not available

1
Azithromycin
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3
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