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Ph. Eur. Azithromycin - Related Substances peak identification solution injection on Gemini™ 3 µm NX-C18 150 x 4.6 mm using Waters® Arc HPLC system

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Application Detail (App ID: 27302)

Column: Gemini® 3 µm NX-C18 110 Å, LC Column 150 x 4.6 mm, Ea

Part No:00F-4453-E0

Dimensions:150 x 4.6 mm ID

Elution Type:Gradient

Elution A:1.80 g/L solution of anhydrous disodium hydrogen phosphate adjusted to pH 8.9

Elution B:Acetonitrile:Methanol = 75:25

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	50	50
2	9	45	55
3	10.8	40	60
4	28.8	25	75
5	29.16	50	50
6	33.48	50	50

Flow Rate:1.667 mL/min

Col. Temp: 60 °C

Detection: LC/UV (DAD, PDA) @ 210.0000000000 nm (nanometers) (0°C)

Note:Diluent buffer: 1.73 g/L solution of ammonium dihydrogen pH adjusted to 10.0 with ammonia
Diluent: Diluent buffer: Acetonitrile: methanol = 35:30:35 (v/v/v)

Analyte Details

Analyte Detail not available

Azithromycin Impurity L

Azithromycin Impurity C

Azithromycin Impurity M

Azithromycin Impurity E

Azithromycin Impurity F

Azithromycin Impurity J

Azithromycin Impurity I

Azithromycin Impurity N

Azithromycin Impurity A

Azithromycin Impurity P

Azithromycin

Azithromycin Impurity O

Azithromycin Impurity G

Azithromycin Impurity B