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2023 MSACL poster LLOQ peak 11 deoxycorticosterone

Sample Preparation Details

Part Number :8E-S100-TGB
Description :Strata™-X 33 µm Polymeric Reversed Phase
Condition 1 :1 mL MeOH
Sample Pretreatment :Dilute 500 uL serum sample with 500 uL 1% FA.
Load :1 mL total volume of pretreated sample and spike with 10uL IS
Wash 1 :1 mL 1% FA
Wash 2 :1 mL 30% MeOH
Dry :Dry down under gentle nitrogen flow at 45 degrees C
Elution 1 :Twice with 500 uL 1:4 MeOH:ACN
Evaporate: :Evaporate to dryness
Reconstitute :100 uL 60:40 0.5mM NH4F (aq) : MeOH
Condition 1 mL MeOH
Equilibrate 1 mL Water
Load 1 mL total volume of pretreated sample and spike with 10uL IS
Wash 1 mL 1% FA
Vacuum Apply vacuum to elute wash
Wash 1 mL 30% MeOH
Vacuum Apply vacuum (15-20") for 3-4 minutes until completely dry
Elute Twice with 500 uL 1:4 MeOH:ACN
Dry Dry down under gentle nitrogen flow at 45 degrees C
Reconstitute 100 uL 60:40 0.5mM NH4F (aq) : MeOH

Application Detail (App ID: 27374)

Part No:00B-4462-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:0.5 mM NH4F (aq)
Elution B:MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 60 40
10 6 5 95
11 6.5 60 40
12 8 60 40
2 2 50 50
8 4.5 25 75
9 5 5 95

Flow Rate:800 μL/min
Col. Temp: 30 °C
Detection: LC/MS/MS @ (700°C)

Analyte Details

Analyte Detail not available