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Optimized method of Valsartan and Sacubitril on Kinetex F5 column

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Application Detail (App ID: 27665)

Column: Kinetex® 5 µm F5 100 Å, LC Column 250 x 4.6 mm, Ea

Part No:00G-4724-E0

Dimensions:250 x 4.6 mm ID

Elution Type:Gradient

Elution A:pH 2.5 10mM potassium dihydrogen phosphate

Elution B:Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	65	35
2	25	65	35
3	32	10	90
4	35	10	90
5	38	65	35
6	45	65	35

Flow Rate:0.8 mL/min

Col. Temp: 40 °C

Detection: UV-Vis Abs.-Diode Array (PDA) @ (0°C)

Analyte Details

Analyte Detail not available

Valsartan

Sacubutril