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Tacrolimus standard solution 30µg/ml. USP-Organic impurities

Application Detail (App ID: 27990)

Part No:00F-4251-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A: Solution -A: - 6mM Phosphoric acid in milliQ water. (0.4ml of 85% Orthophosphoric acid in 1000ml of water, filtered through 0.22µm membrane nylon filter.) Solution- B: - A Mixture of Acetonitrile tert-butyl methyl ether in ratio of 810:190ml v/v. Mobile phase A: Solution A and Solution B in a ratio of 4:1 (400:100 ml v/v)
Elution B: Solution -A: - 6mM Phosphoric acid in milliQ water. (0.4ml of 85% Orthophosphoric acid in 1000ml of water, filtered through 0.22µm membrane nylon filter.) Solution- B: - A Mixture of Acetonitrile tert-butyl methyl ether in ratio of 810:190ml v/v. Mobile phase B: Solution A and Solution B in a ratio of 1:4 (400:400 ml v/v)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 72 28
2 30 72 28
3 53 15 85
4 54 72 28
5 60 72 28

Flow Rate:1.5 mL/min
Col. Temp: 60 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 220.0000000000 nm (nanometers) (0°C)
Note:1st Individual chromatogram of Standard solution 6-replicate injections

Analyte Details

Analyte Detail not available

1
Tacrolimus 19-epimer
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2
Tacrolimus
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3
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4
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