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USP Abacavir and Lamivudine Tablets - Assay (Standard Solution)

Application Detail (App ID: 28077)

Part No:00F-4784-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:Water and TFA (2000:1)
Elution B:Acetonitrile, Methanol and TFA (1000:1000:1)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 3.43 100 0
3 10.29 70 30
4 10.37 40 60
5 11.23 40 60
6 11.31 100 0
7 18.31 100 0

Flow Rate:1.5 mL/min
Col. Temp: 40 °C
Detection: LC/UV (DAD, PDA) @ 270.0000000000 nm (nanometers) (0°C)
Note:0.35 mg/mL of USP Abacavir Sulfate RS and 0.15 mg/mL of USP Lamivudine RS in 0.1N hydrochloric acid. Sonicate to dissolve prior to final dilution. Pass through a Nylon filter (Clarify Syringe filter PN: AF8-7707-12)

Analyte Details

Analyte Detail not available

1
Lamivudine
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2
Abacavir
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