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Dabsyl Derivated Phospho Amino Acids

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Application Detail (App ID: 5642)

Column: Ultracarb™ 5 µm ODS (30) 60 Å, LC Column 250 x 4.6 mm, Ea

Part No:00G-0351-E0

Dimensions:250 x 4.6 mm ID

Elution Type:Gradient

Elution A:20 mM Sodium bicarbonate pH 8.1 with 4% dimethylformamide

Elution B:(90:10) Acetonitrile/2-Propanol

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	82	18
2	6	75	25
3	26	60	40
4	41	30	70
5	43	30	70

Flow Rate:1 mL/min

Col. Temp: ambient

Detection: UV-Vis Abs.-Variable Wave.(UV) @ 460 nm (22°C)

Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Phosphothreonine

Phosphoserine

Phosphotyrosine

Aspartic acid

Glutamic acid

Carboxymethylcysteine

Serine

Threonine

Glycine

Alanine

Proline

Valine

Methionine

Isoleucine

Leucine

Arginine

Phenylalanine

Cysteine

Ammonia

Lysine

Histidine

Dityrosine

Tyrosine