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What is the most common UV wavelength to detect proteins?

APPLICATION PROTOCOL

AERIS BIOSEP-SEC-S JUPITER YARRA HPLC UHPLC REVERSED PHASE SIZE EXCLUSION LIFE SCIENCE PHARMA

Last Updated: 11/17/2014

The most common wavelength used for detection of proteins is 280 nm. Absorption of radiation in the near UV by proteins depends on the Tyr and Trp content (and to a very small extent on the amount of Phe and disulfide bonds) at this wavelength