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What are the recommended long term storage conditions for HPLC columns?

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HPLC UHPLC

Last Updated: 12/2/2014

Column storage conditions can affect column lifetime. As such, long term storage of columns containing buffers or ion-pairing reagents is not recommended.

Before storing, flush with 5 column volumes of mobile phase without buffer to remove any buffers or salts. Refer to the table below for recommended storage conditions based upon the chemistry of your column. 

Column Type Storage Solvent
Reversed Phase
C18, C12, C8, C4, C2, C1,
Phenyl, PFP
65 % Acetonitrile/
35 % Water
Normal Phase
Silica, CN, NH2, PAC,
Diol Alumina
Isopropanol or Hexane
Ion-Exchange
SAX, SCX, WAX, WCX
Methanol*
Size-Exclusion
Diol
0.05 % NaN3 in water or
10 % methanol
HILIC
Luna HILIC
80 % Acetonitrile/
20 % Water