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Column storage conditions can affect column lifetime. As such, long term storage of columns containing buffers or ion-pairing reagents is not recommended.
Before storing, flush with 5 column volumes of mobile phase without buffer to remove any buffers or salts. Refer to the table below for recommended storage conditions based upon the chemistry of your column.
Column Type | Storage Solvent |
Reversed Phase C18, C12, C8, C4, C2, C1, Phenyl, PFP |
65 % Acetonitrile/ 35 % Water |
Normal Phase Silica, CN, NH2, PAC, Diol Alumina |
Isopropanol or Hexane |
Ion-Exchange SAX, SCX, WAX, WCX |
Methanol* |
Size-Exclusion Diol |
0.05 % NaN3 in water or 10 % methanol |
HILIC Luna HILIC |
80 % Acetonitrile/ 20 % Water |