Jupiter HPLC Information

RP-HPLC for Protein/Peptide Analysis and Purification

Jupiter^® HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification.

Jupiter 300 - 300 Å columns are designed to analyze and purify intact proteins

Juptier Proteo - 90 Å columns are engineered for increased peak capacity and resolution of peptide maps as well as peptide separations

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<p style="cursor: auto;"><strong style="cursor: auto;">RP-HPLC for Protein/Peptide Analysis and Purification</strong></p>

<p style="cursor: auto;">Jupiter<sup style="cursor: auto;">®</sup> HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification.</p>

<p style="cursor: auto;"><strong style="cursor: auto;">Jupiter 300 - 300 Å</strong> columns are designed to analyze and purify intact proteins</p>

<p style="cursor: auto;"><strong style="cursor: auto;">Juptier Proteo - 90 Å</strong> columns are engineered for increased peak capacity and resolution of peptide maps as well as peptide separations</p>
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4 Phases

Reversed Phase 4

C18

Particle Sizes (µm)	3, 5, 10, 15
Pore Size (Å)	300
Surface Area (sq. m/g)	170
Carbon Load (%)	13.34

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C18 Phase Information

Jupiter 300 C18 columns are excellent for polar as well as non-polar intact proteins, and is the most retentive of all the Jupiter phases. This increased retention allows one to separate proteins with only slight differences in hydrophobicity.

For separation of intact proteins >10,000 MW
Great for hydrophilic proteins
Direct scale-up to preparative and bulk materials
300 Å also available in C4 and C5 bonded phases

4 Phases

Reversed Phase 4

Technical Documents
Technical Specifications
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Phase	Particle Sizes (µm)	Pore Size (Å)	Surface Area (sq. m/g)	Carbon Load (%)	Recommended Use
C18	3, 5, 10, 15	300	170	13.34	Separation of hydrophilic proteins > 10,000 Da
C4	5, 10, 15	300	170	5	Separation of highly hydrophobic proteins > 10,000 Da
C5	5, 10	300	170	5.5	Separation of highly hydrophobic proteins > 10,000 Da; Slightly different selectivity than C4
Proteo	4, 10	90	475	15	Purification of synthetic peptide mixtures; Characterization of proteins by peptide mapping

Document Type:

Document Type	Title
Brochures	Axia- The Ultimate Pre-packed Preparative Column	Add to My Documents
Other	Bulk Media Selection Guide	Add to My Documents
Brochures	Capillary and Trap Column Solutions for Microflow LC	Add to My Documents
Other	Chromatography Bulk Media and PREP Guide	Add to My Documents
MSDS	Jupiter 10 μm C18	Add to My Documents
MSDS	Jupiter 10 μm C4	Add to My Documents
MSDS	Jupiter 15 μm C18	Add to My Documents
MSDS	Jupiter 15 μm C4	Add to My Documents
Supporting Docs	Jupiter Column Care Note	Add to My Documents
Technical Notes	Interferon-α Protein Modifications by HPLC (TN-1053)	Add to My Documents
Other	Pharmaceutical Analysis Guide- Formulations	Add to My Documents
Brochures	Reversed Phase HPLC Solutions for Proteins and Peptides	Add to My Documents
Brochures	SecurityGuard Column Protection Family Brochure (UHPLC, HPLC, PREP, SFC)	Add to My Documents
Supporting Docs	Troubleshooting for HPLC: Recognizing and Anticipating Challenges during Reversed Phase HPLC	Add to My Documents
Technical Notes	PEGylated Proteins by Reversed Phase HPLC (TN-1046)	Add to My Documents
MSDS	Safety Data Sheet for Silica-Based HPLC Columns	Add to My Documents
Other	USP Allowable Adjustments Guide	Add to My Documents
Other	USP Allowable Adjustments Guide Japanese (米国薬局方（USP)メソッドの許容調整)	Add to My Documents
Technical Notes	PEGylated Proteins by HPLC-UV (TN-1034)	Add to My Documents
Supporting Docs	Meeting All Your PREP Needs	Add to My Documents
Other	Protein and Peptide Users Guide	Add to My Documents
Supporting Docs	HPLC Column Care Guide	Add to My Documents

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Part Number	Brand	Phase	Particle Size (µm)	Pore Size (Å)	Length (mm)	Internal Diameter (mm)	Price

Jupiter Columns

Jupiter HPLC Information

C18

C18 Phase Information