Yarra HPLC Information

Better Protein/Peptide Gel Filtration Separations Start Here

Meet

Coupled with tight particle and pore size distribution as well as strict packing and QC specifications, Yarra SEC/GFC columns allow Biochromatographers to achieve the highest efficiencies and resolution possible for their biomolecule analysis.

Better Recovery of Biomolecules

^New Yarra 1.8µm SEC-X150

Extremely inert 1.8µm particle with LMW separation range of 1K-450KDa for monoclonal antibody (mAb) fragment analysis, peptide characterization, and biosimilars.

^New Yarra 1.8µm SEC-X300

Extremely inert 1.8µm particle with HMW separation range of 10K-700KDa for separating and characterizing mAb aggregates, antibody drug conjugates (ADCs) and biosimilars.

Ultra High Resolution from Analytical to Prep Scale

3µ

Recommended for analytical purification, desalting, and characterization of biomolecules

View Speciifications »

5µ

Recommended for preparative purification, desalting, and characterization of biomolecules

View Speciifications »

Characteristic	Yarra SEC-2000 PREP	Yarra SEC-3000 PREP	Yarra SEC-4000 PREP
Resin	Silica	Silica	Silica
Particle Size (µm)	5	5	5
Pore Size (Å)	145	290	500
pH Range	2.5-7.5	2.5-7.5	2.5-7.5
Maximum Backpressure (psi)	1,500	1,500	1,500
Typical Backpressure (psi)	800	800	800
Efficiency (min. plates, 300 x 21.2 mm)	30,000	30,000	30,000
Maximum Flow Rate	10 mL/min	10 mL/min	10 mL/min

Characteristic	Yarra SEC-2000	Yarra SEC-3000	Yarra SEC-4000
Resin	Silica	Silica	Silica
Particle Size (µm)	3	3	3
Pore Size (Å)	145	290	500
pH Range	2.5-7.5	2.5-7.5	2.5-7.5
Maximum Backpressure (psi)	3,000	3,000	1,700
Typical Backpressure (psi)	1,300	1,300	1,000
Efficiency (min. plates, 300 x 7.8 mm)	48,000	48,000	38,000
Maximum Flow Rate	1.5 mL/min	1.5 mL/min	1.2 mL/min

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<section id="ycontain">
<h2 class="ytitle" style="color:#552A2E">Better Protein/Peptide Gel Filtration Separations Start Here</h2>

<div class="ydetail">
<p>Coupled with tight particle and pore size distribution as well as strict packing and QC specifications, Yarra SEC/GFC columns allow Biochromatographers to achieve the <strong>highest efficiencies and resolution possible</strong> for their biomolecule analysis.</p>
</div>

<h2 class="ytitle ytop" style="color:#552A2E">Better Recovery of Biomolecules</h2>

<div class="ymcard">
<div class="ymarker-three">
<p class="ynew"><sup><span style="font-size:11px">New</span></sup> Yarra 1.8µm SEC-X150</p>
</div>

<div class="ydetail-three">
<p>Extremely inert 1.8µm particle with LMW separation range of 1K-450KDa for monoclonal antibody (mAb) fragment analysis, peptide characterization, and biosimilars.</p>
</div>

<div class="ymarker-three-hundred" style="background-color:#478538">
<p class="ynew"><sup><span style="font-size:11px">New</span></sup> Yarra 1.8µm SEC-X300</p>
</div>

<div class="ydetail-three-hundred">
<p>Extremely inert 1.8µm particle with HMW separation range of 10K-700KDa for separating and characterizing mAb aggregates, antibody drug conjugates (ADCs) and biosimilars.</p>
</div>

<h2 class="ytitle ytop" style="color:#552A2E">Ultra High Resolution from Analytical to Prep Scale</h2>

<div class="ydetail-two">
<p>Recommended for analytical purification, desalting, and characterization of biomolecules</p>
<a class="btn btn-small btn-primary" data-toggle="modal" href="#three" style="margin-left:5px;">View Speciifications »</a></div>

<div class="ydetail-two">
<p>Recommended for preparative purification, desalting, and characterization of biomolecules</p>
<a class="btn btn-small btn-primary" data-toggle="modal" href="#five" style="margin-left:5px;">View Speciifications »</a></div>

<h1>Yarra 5µm GFC/SEC Columns</h1>
</div>

<div class="modal-body;" style="height:475px;">
<div class="mg-faq">
<table class="table table-striped" style="width:100%;">
	<tbody style="cursor: auto;">
		<tr>
			<th style="width: 280px;">Characteristic</th>
			<th style="width: 140px;">Yarra SEC-2000 PREP</th>
			<th style="width: 140px;">Yarra SEC-3000 PREP</th>
			<th style="width: 140px;">Yarra SEC-4000 PREP</th>
		</tr>
		<tr>
			<td>Resin</td>
			<td>Silica</td>
			<td>Silica</td>
			<td>Silica</td>
		</tr>
		<tr>
			<td>Particle Size (µm)</td>
			<td>5</td>
			<td>5</td>
			<td>5</td>
		</tr>
		<tr>
			<td>Pore Size (Å)</td>
			<td>145</td>
			<td>290</td>
			<td>500</td>
		</tr>
		<tr>
			<td>pH Range</td>
			<td>2.5-7.5</td>
			<td>2.5-7.5</td>
			<td>2.5-7.5</td>
		</tr>
		<tr>
			<td>Maximum Backpressure (psi)</td>
			<td>1,500</td>
			<td>1,500</td>
			<td>1,500</td>
		</tr>
		<tr>
			<td>Typical Backpressure (psi)</td>
			<td>800</td>
			<td>800</td>
			<td>800</td>
		</tr>
		<tr>
			<td>Efficiency (min. plates, 300 x 21.2 mm)</td>
			<td>30,000</td>
			<td>30,000</td>
			<td>30,000</td>
		</tr>
		<tr>
			<td>Maximum Flow Rate</td>
			<td>10 mL/min</td>
			<td>10 mL/min</td>
			<td>10 mL/min</td>
		</tr>
	</tbody>
</table>
</div>
</div>
</div>

<h1>Yarra 3µm GFC/SEC Columns</h1>
</div>

<div class="modal-body;" style="height:475px;">
<div class="mg-faq">
<table class="table table-striped" style="width:100%">
	<tbody style="cursor: auto;">
		<tr>
			<th>Characteristic</th>
			<th>Yarra SEC-2000</th>
			<th>Yarra SEC-3000</th>
			<th>Yarra SEC-4000</th>
		</tr>
		<tr>
			<td>Resin</td>
			<td>Silica</td>
			<td>Silica</td>
			<td>Silica</td>
		</tr>
		<tr>
			<td>Particle Size (µm)</td>
			<td>3</td>
			<td>3</td>
			<td>3</td>
		</tr>
		<tr>
			<td>Pore Size (Å)</td>
			<td>145</td>
			<td>290</td>
			<td>500</td>
		</tr>
		<tr>
			<td>pH Range</td>
			<td>2.5-7.5</td>
			<td>2.5-7.5</td>
			<td>2.5-7.5</td>
		</tr>
		<tr>
			<td>Maximum Backpressure (psi)</td>
			<td>3,000</td>
			<td>3,000</td>
			<td>1,700</td>
		</tr>
		<tr>
			<td>Typical Backpressure (psi)</td>
			<td>1,300</td>
			<td>1,300</td>
			<td>1,000</td>
		</tr>
		<tr>
			<td>Efficiency (min. plates, 300 x 7.8 mm)</td>
			<td>48,000</td>
			<td>48,000</td>
			<td>38,000</td>
		</tr>
		<tr>
			<td>Maximum Flow Rate</td>
			<td>1.5 mL/min</td>
			<td>1.5 mL/min</td>
			<td>1.2 mL/min</td>
		</tr>
	</tbody>
</table>
</div>
</div>
</div>
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6 Phases

Gel Filtration 6

Featured

BROCHURE

Yarra GFC/SEC Column Product Guide

Biomolecule characterization and purification at extremely affordable prices
VIDEO

On-Demand GFC Webinar with The Analytical Scientist

GFC for protein aggregation and protein post-translational modifications (PTMs)
GUIDE

Online BioSeparations Handbook

Optimize HPLC|UHPLC and GFC separations for proteins, peptides, and other biomolecules.
TECHNICAL NOTE

Analysis of Antibody Drug Conjugates (ADCs) using High Efficiency Gel Filtration Columns

In this study, we investigate the feasibility of using a non-toxic ADC model with a Yarra 3 μm SEC-3000 column for aggregate analysis.

SEC-4000

Particle Sizes (µm)	3
Pore Size (Å)	500

Compare All Phases

SEC-4000 Phase Information

Use Yarra SEC-4000 for

Large proteins
Monoclonal antibodies
PEGylated IgG

Want Even Longer Column Lifetimes for Yarra?

Try SecurityGuard ™ Universal Guard Cartridge System.

6 Phases

Gel Filtration 6

Technical Documents
Technical Specifications
Search FAQ

Phase	Particle Sizes (µm)	Pore Size (Å)
SEC-2000	3	145
SEC-2000 PREP	5	145
SEC-3000	3	290
SEC-3000 PREP	5	290
SEC-4000	3	500
SEC-4000 PREP	5	500

Document Type:

Document Type	Title
Technical Notes	Low Molecular Weight (MW) Biomolecule Separation with a Yarra 1.8 μm SEC-X150 Column	Add to My Documents
Technical Notes	Mobile Phase Optimization for Aggregate Analysis of Monoclonal Antibodies (TN-1226)	Add to My Documents
Webinar	On-Demand BioSeparations Webinar from Phenomenex and C&E News	Add to My Documents
Brochures	Online BioSeparations Handbook	Add to My Documents
Other	Pharmaceutical Analysis Guide- Formulations	Add to My Documents
Technical Notes	Polysorbate 80 (Tween 80) HPLC-UV Analysis on a Yarra 1.8 μm SEC-X150 Aqueous GFC/SEC Column	Add to My Documents
Brochures	SecurityGuard Column Protection Family Brochure (UHPLC, HPLC, PREP, SFC)	Add to My Documents
Technical Notes	Size Exclusion Chromatography Method Optimization using the European Pharmacopoeia for Insulin Fibrils (Ph. Eur. Monograph 838) (TN-1188)	Add to My Documents
Technical Notes	Size Exclusion Chromatography Optimization using the European Pharmacopeia Method for Follitropin Oligomers (TN-1204)	Add to My Documents
Webinar	Webinar White Paper: Transform Your Mindset for HPLC and UHPLC Method Development For Analyzing Proteins- Including Monoclonal Antibodies	Add to My Documents
Technical Notes	Analysis of Antibody Drug Conjugates (ADCs) using High Efficiency Gel Filtration Columns (TN-1176)	Add to My Documents
Technical Notes	Better Gel Filtration: A New Look at Method Development Parameters (TN-1156)	Add to My Documents
Technical Notes	Better Sample Recovery with Yarra SEC-X150	Add to My Documents
Technical Notes	Impressive Yarra 1.8 μm SEC-X150 Column Stability after 100 Hours of Harsh Conditions	Add to My Documents
Technical Notes	Relevant Parameters in Developing Protein Aggregation Methods Using Yarra GFC Columns (TN-1131)	Add to My Documents
Other	USP Allowable Adjustments Guide	Add to My Documents
Other	USP Allowable Adjustments Guide Japanese (米国薬局方（USP)メソッドの許容調整)	Add to My Documents
Brochures	Yarra GFC/SEC Column Product Guide	Add to My Documents
Supporting Docs	Meeting All Your PREP Needs	Add to My Documents
Other	Protein and Peptide Users Guide	Add to My Documents
Supporting Docs	Yarra Care and Use Notes	Add to My Documents
Supporting Docs	HPLC Column Care Guide	Add to My Documents

Phase: Particle Size: Pore Size: Length: Internal Diameter:

Part Number:

Description:

Required Holder:

Stationary Phase:

Solid Support:

Format:

Separation Mode:

Recommended Use:

USP Designation:

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Part Number	Brand	Phase	Particle Size (µm)	Pore Size (Å)	Length (mm)	Internal Diameter (mm)	Price

Ultra-High Resolution Aqueous Size

Exclusion at an Unbelievable Price

Yarra HPLC Information

Better Protein/Peptide Gel Filtration Separations Start Here

Better Recovery of Biomolecules

Ultra High Resolution from Analytical to Prep Scale

Featured

Yarra GFC/SEC Column Product Guide

On-Demand GFC Webinar with The Analytical Scientist

Online BioSeparations Handbook

Analysis of Antibody Drug Conjugates (ADCs) using High Efficiency Gel Filtration Columns

SEC-4000

SEC-4000 Phase Information