Gemini HPLC Information

U.S. Patent No. 7, 563, 367

Setting the Standard for pH Method Development

Rugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations.

Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity
Expect longer column lifetime with patented TWIN-NX^™ technology
Ideal for analytical and preparative separations of basic and acidic compounds

Inside Twin Technology

During the final stage of silica manufacturing a unique silica-organic layer is grafted to create a completely new composite particle. This composite particle particle retains its mechanical strength and rigidity along with excellent efficiency, while the silica-organic shell protects the particle from chemical attack.

Inside Twin-NX Technology

TWIN-NX technology uses an improved patented organo-silica grafting process which incorporates highly stabilizing ethane crosslinking.

Gemini C18 and C6-Phenyl
TWIN Technology

Gemini NX-C18
TWIN-NX Technology

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<div id="twinpg">
<p style="cursor: auto;">U.S. Patent No. 7, 563, 367</p>

<p style="cursor: auto;"><strong style="cursor: auto;">Setting the Standard for pH Method Development</strong></p>

<p style="cursor: auto;">Rugged <strong>reversed phase HPLC columns</strong> that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations.</p>

<ul class="gemlist">
	<li style="cursor: auto;">Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity</li>
	<li style="cursor: auto;">Expect longer column lifetime with patented TWIN-NX<sup style="cursor: auto;">™</sup> technology</li>
	<li style="cursor: auto;">Ideal for analytical and preparative separations of basic and acidic compounds</li>
</ul>

<div id="twintechcard">
<h2>Inside Twin Technology</h2>

<p>During the final stage of silica manufacturing a unique silica-organic layer is grafted to create a completely new composite particle. This composite particle particle retains its mechanical strength and rigidity along with excellent efficiency, while the silica-organic shell protects the particle from chemical attack.</p>
</div>

<div id="twinnxtechcard">
<h2>Inside Twin-NX Technology</h2>

<p>TWIN-NX technology uses an improved patented organo-silica grafting process which incorporates highly stabilizing ethane crosslinking.</p>
</div>

<div class="gemcard" id="twin-tech">
<h2 style="line-height:18px; padding-left:20px">Gemini C18 and C6-Phenyl<br />
<span style="color:#000">TWIN Technology</span></h2>
<img src="/webimage/16twin_graphic.png" /></div>

<div class="gemcard" id="nx-tech">
<h2 style="line-height:18px;padding-left:20px">Gemini NX-C18<br />
<span style="color:#000">TWIN-NX Technology</span></h2>
<img src="/webimage/16twinnx_graphic.png" /></div>
</div>
<script src="//cdnjs.cloudflare.com/ajax/libs/gsap/latest/TweenMax.min.js"></script><script src="//phenomenex.blob.core.windows.net/cdn/cdnscripts/brandgeminimg.js"></script>

4 Phases

Reversed Phase 4

Featured

POSTER

Control the Selectivity of Acids and Bases with pH

Take advantage of the selectivity benefits at fringe pH conditions with the highly pH stable Gemini columns
BROCHURE

pH Flexibility Expands Robustness and Reproducibility

Rugged reversed phase HPLC columns that offer extended lifetime at extreme pH conditions and excellent stability for reproducible, high efficiency separations
TECHNICAL NOTE

LC/MS/MS Analysis of Mycotoxins and Other Fungal/Bacteria Metabolites

Sensitive and fast analysis of over 240 mycotoxins and other biological contaminants
TECHNICAL NOTE

Difficult Aromatic Separations with Gemini C6-Phenyl

Utilize the high efficiency and excellent aromatic selectivity of Gemini C6-Phenyl for complex separations

NX-C18

Particle Sizes (µm)	3, 5, 10
Pore Size (Å)	110
Surface Area (sq. m/g)	375
Carbon Load (%)	14

Compare All Phases

NX-C18 Phase Information

The most rugged and highly efficient Gemini phase.

Control selectivity of ionizable compounds for optimized methods
Consistent performance in both volatile and non-volatile buffers
High and low pH methods

4 Phases

Reversed Phase 4

Technical Documents
Technical Specifications
Search FAQ

Phase	Particle Sizes (µm)	Pore Size (Å)	Surface Area (sq. m/g)	Carbon Load (%)	Recommended Use
C18	3, 5, 10	110	375	14	Separation of basic compounds under high pH conditions
C6-Phenyl	3, 5	110	375	12	Separation of aromatic, polar, or basic compounds under high pH conditions
C8(3)	10	100		N/A
NX-C18	3, 5, 10	110	375	14	High efficiency analytical or preparative separations under extreme pH conditions

Document Type:

Document Type	Title
Technical Notes	Achieving Low Parts-per-Quadrillion Detection Limits for PFAS Analysis in Drinking Water (TN-1316)	Add to My Documents
Technical Notes	Analysis of PFAS in Drinking Water by EPA Method 533: A Direct Comparison of the Accuracy and Precision of Manual and Automated SPE Sample Preparation (TN-0151)	Add to My Documents
Brochures	Axia- The Ultimate Pre-packed Preparative Column	Add to My Documents
Other	Bulk Media Selection Guide	Add to My Documents
Brochures	Capillary and Trap Column Solutions for Microflow LC	Add to My Documents
Other	Chemical Industry LC & GC Separation Solutions Guide	Add to My Documents
Other	Chromatography Bulk Media and PREP Guide	Add to My Documents
Supporting Docs	Clinical Resources for Toxicology Analysis	Add to My Documents
Technical Notes	Determination of Perfluoroalkyl Substances (PFAS) in Sediments by QuEChERS and LC-MS/MS (TN-0117)	Add to My Documents
Other	Environmental Applications Guide	Add to My Documents
Technical Notes	EPA Method 533: PFAS in Drinking Water	Add to My Documents
Technical Notes	Evaluation of Extraction Options for EPA Draft Method 1633	Add to My Documents
Brochures	Extend your Analytical and Preparative HPLC/ SFC Column Lifetimes	Add to My Documents
Technical Notes	Extended Gemini C18 Column Lifetime Using SecurityGuard	Add to My Documents
Technical Notes	Extended Gemini NX-C18 Column Lifetime Using SecurityGuard	Add to My Documents
MSDS	Gemini 10 μm C8(3)	Add to My Documents
MSDS	Gemini 10 μm NX-C18	Add to My Documents
MSDS	Gemini 3 μm NX-C18	Add to My Documents
MSDS	Gemini 5 μm NX-C18	Add to My Documents
Supporting Docs	Gemini Care and Use Notes	Add to My Documents
Technical Notes	Glyphosate Analysis by LC/MS/MS using FMOC Derivitization or Underivitized Method	Add to My Documents
Technical Notes	Hormones from Drinking Water by LC/MS/MS (TN-1163)	Add to My Documents
Technical Notes	Vitamin C from Tablets and Soft-Gels by HPLC-UV (TN-1153)	Add to My Documents
Other	HPLC/UHPLC Column Phase Selection Chart	Add to My Documents
Brochures	Improve Your European Pharmacopoeia (Ph. Eur.) and United States (USP) Monographs	Add to My Documents
Technical Notes	Basic Compounds by LC/MS using a High pH Mobile Phase (TN-1031)	Add to My Documents
Supporting Docs	Modern Approaches for PFAS LC-MS/MS Analysis in Aqueous and Solid Matric	Add to My Documents
Technical Notes	Modernization of USP and Ph. Eur. Method – Pantoprazole Sodium Sesquihydrate Organic Impurities	Add to My Documents
Brochures	New pH Stable Media for Maximum Lifetime in Preparative Purifications	Add to My Documents
Brochures	Pain Panel Research and Solutiuons Brochure	Add to My Documents
Technical Notes	Per and Polyfluoroalkyl Substances (PFAS) Extraction by LC MS/MS Using Strata PFAS for a Stacked Solid Phase Extraction (SPE) Solution	Add to My Documents
Brochures	Pesticide Analysis by GC/LC/Sample Prep	Add to My Documents
Technical Notes	PFAS - Preventing Sorbent Overload in PFAS Drinking Water Analysis using EPA 533 Method	Add to My Documents
Technical Notes	PFAS Analysis in Water Samples using LC/MS/MS Large-Volume Direct Injection	Add to My Documents
Brochures	PFAS Analytical Workflow Solutions	Add to My Documents
Other	PFAS Complete Testing Guide	Add to My Documents
Other	PFAS in Drinking Water (Extract from PFAS Guide)	Add to My Documents
Other	PFAS in Wastewater, Sediment, and Soils	Add to My Documents
Brochures	PFAS Q&A	Add to My Documents
Brochures	pH Flexibility Expands Robustness and Reproducibility	Add to My Documents
Technical Notes	Ph. Eur. Monograph 0923: Azithromycin Related Substances on Gemini NX-C18 and Durashell C18(L) Columns	Add to My Documents
Technical Notes	Ph. Eur. Monograph 1368: Ramipril Related Substances on NUCLEOSIL® 3 µm C18, Luna ™3 µm C18(2), Luna Omega 3 µm C18, and Gemini ™3 µm NX C18 Column	Add to My Documents
Other	Pharmaceutical Analysis Guide- Formulations	Add to My Documents
Brochures	Phenova CRMs Product Brochure	Add to My Documents
Technical Notes	Potassium Clavulanate Ph. Eur Monograph	Add to My Documents
Technical Notes	Low Concentration Amphetamines by SPE and LC/MS/MS (TN-0020)	Add to My Documents
Technical Notes	Rapid LC-MS-MS Method for Monitoring Bio-Relevant Levels of PFAS in Serum (TN-1325)	Add to My Documents
Technical Notes	Reducing PFAS Interferences During Human Plasma and Serum Analysis with Accurate Mass Spectrometry (TN-1323)	Add to My Documents
Brochures	SecurityGuard Column Protection Family Brochure (UHPLC, HPLC, PREP, SFC)	Add to My Documents
Technical Notes	Significance of HPLC in the Development and Production of the Antiviral Drug Remdesivir	Add to My Documents
Technical Notes	Stationary Phases for the Process Scale Purification of Peptides and Insulin Analogs	Add to My Documents
Other	THE Clinical Application Notebook, Volume 2	Add to My Documents
Other	The Complete Guide to Solid Phase Extraction (SPE)	Add to My Documents
Other	The Ultimate Guide to HPLC/UHPLC Reversed Phase Selectivity	Add to My Documents
Other	Ultimate Guide to Reversed Phase HPLC/UHPLC - Column Selectivity Selection Poster	Add to My Documents
Other	USP and EP Monograph Guide	Add to My Documents
Brochures	Vitamin Testing Brochure	Add to My Documents
Technical Notes	Basic Compounds by LC/MS using High pH Mobile Phase (TN-1031)	Add to My Documents
Technical Notes	A Simple and Effective High pH LC-MS/MS Method for Determination of Underivatized Vitamin B1 and B6 in Human Whole Blood (TN-1205)	Add to My Documents
Technical Notes	AN-1010_USP Alosetron Hydrochloride Assay and Organic Impurities on Gemini® 3 µm C18	Add to My Documents
Technical Notes	AN-1011_USP Alosetron Tablets Assay and Organic Impurities on Gemini® 3 µm C18	Add to My Documents
Technical Notes	AN-1014: USP Vardenafil Hydrochloride Assay and Organic Impurities on Gemini® 5 µm C18 and Gemini 5 µm NX-C18	Add to My Documents
Technical Notes	Antibiotics Screen from Bovine Kidney Juice by LC/MS/MS (TN-1117)	Add to My Documents
Brochures	Axia Packed Gemini-NX Preparative Columns	Add to My Documents
Supporting Docs	Clinical Resources for Vitamin Testing	Add to My Documents
Supporting Docs	Hardware Change Insert	Add to My Documents
Technical Notes	Sulfonamides from Honey by LC/MS/MS (TN-0012)	Add to My Documents
Technical Notes	Steroids from Urine and Plasma by SPE and LC/MS/MS (TN-1026)	Add to My Documents
Technical Notes	Acetaminophen, Phenylephrine, Chlorpheneramine and Dextromethorphan by HPLC-UV (TN-1044)	Add to My Documents
Technical Notes	Mycotoxins and Other Fungal/Bacterial Metabolites from Several Food Matrices by LC/MS/MS (TN-1119)	Add to My Documents
Technical Notes	Nicotine, Cotinine, 3-Hydroxycotinine, Nornicotine and Anabasine in Human Urine by LC/MS/MS (TN-1161)	Add to My Documents
Technical Notes	PFAS and GenX from Water by Solid Phase Extraction and LC-MS/MS	Add to My Documents
Supporting Docs	pH LC Poster	Add to My Documents
Technical Notes	Ph.Eur. Monograph 0721: Ibuprofen Related Substances on Fully Porous and Core-Shell Organo-silica HPLC Columns	Add to My Documents
MSDS	Safety Data Sheet for Silica-Based HPLC Columns	Add to My Documents
Technical Notes	Nicotinic Acid and Nicotinamide in Human Plasma by LC/MS/MS (TN-1151)	Add to My Documents
Technical Notes	Thiamine Monophosphate (TMP) and Thiamine from Human Breast Milk (TN-0062)	Add to My Documents
Technical Notes	Using pH-LC to Control Selectivity of Acidic and Basic Compounds by HPLC (TN-1042)	Add to My Documents
Other	USP Allowable Adjustments Guide	Add to My Documents
Other	USP Allowable Adjustments Guide Japanese (米国薬局方（USP)メソッドの許容調整)	Add to My Documents
Webinar	Webinar Highlights: Workflow Considerations for Advanced PFAS/GenX LC-MS/MS Sample Preparation and Chromatography	Add to My Documents
Other	Allowable Adjustments to European Pharmacopoeia (EP) Methods	Add to My Documents
Technical Notes	Glyphosate Analysis by LC/MS/MS Using Gemini (FMOC Derivatized)	Add to My Documents
Technical Notes	Impact of pH on Improving Purity and Yield for Preparative Separations (TN-1050)	Add to My Documents
Technical Notes	Impact of pH on Improving Purity and Yield for Preparative Separations (TN-1050)	Add to My Documents
Technical Notes	Increase Column Performance and Lifetime in Peptide and Protein Purification using Aggressive Wash Conditions (TN-1138)	Add to My Documents
Supporting Docs	Meeting All Your PREP Needs	Add to My Documents
Supporting Docs	Meeting All Your SFC Needs	Add to My Documents
Technical Notes	Novel Approach to Separating Amphetamine Enantiomers from Urine Using Lux® 3 μm AMP Chiral Column	Add to My Documents
Technical Notes	Peptide Purification Method Development: Application for the Purification of Bivalirudin (TN-1129)	Add to My Documents
Technical Notes	Using Aromatic Selectivity for Difﬁcult HPLC Separations (TN-1029)	Add to My Documents
Other	Perfluoroalkyl Substances (PFAS) Application Guide - Japanese	Add to My Documents
Supporting Docs	Axia Packed Columns Vs Traditional Packed Preparative Columns (Video)	Add to My Documents
Supporting Docs	HPLC Column Care Guide	Add to My Documents
Other	HPLC Selectivity Poster - LC ツールボックスの選択性を拡大	Add to My Documents
Other	HPLCトラブルシューティングガイド - HPLC Troubleshooting Guide	Add to My Documents

Phase: Particle Size: Pore Size: Length: Internal Diameter:

Part Number:00D-4454-B0

Description:Gemini® 5 µm NX-C18 110 Å, LC Column 100 x 2 mm, Ea

Required Holder:

Stationary Phase:

Solid Support:Fully Porous Organo-silica

Format:Column

Separation Mode:Reversed Phase

Recommended Use:High efficiency analytical or preparative separations under extreme pH conditions

USP Designation:L1

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Part Number	Brand	Phase	Particle Size (µm)	Pore Size (Å)	Length (mm)	Internal Diameter (mm)	Price

Rugged, pH stable HPLC Columns

Gemini HPLC Information

Inside Twin Technology

Inside Twin-NX Technology

Gemini C18 and C6-Phenyl TWIN Technology

Gemini NX-C18 TWIN-NX Technology

Featured

Control the Selectivity of Acids and Bases with pH

pH Flexibility Expands Robustness and Reproducibility

LC/MS/MS Analysis of Mycotoxins and Other Fungal/Bacteria Metabolites

Difficult Aromatic Separations with Gemini C6-Phenyl

NX-C18

NX-C18 Phase Information

Gemini C18 and C6-Phenyl
TWIN Technology

Gemini NX-C18
TWIN-NX Technology