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Jupiter Columns

Jupiter HPLC Information


RP-HPLC for Protein/Peptide Analysis and Purification

Jupiter® HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification.

 

Jupiter 300 - 300 Å columns are designed to analyze and purify intact proteins

 

Juptier Proteo - 90 Å columns are engineered for increased peak capacity and resolution of peptide maps as well as peptide separations

C18


Particle Sizes (µm)3, 5, 10, 15
Pore Size (Å)300
Surface Area (sq. m/g)170
Carbon Load (%)13.34
Compare All Phases

C18 Phase Information


Jupiter 300 C18 columns are excellent for polar as well as non-polar intact proteins, and is the most retentive of all the Jupiter phases.  This increased retention allows one to separate proteins with only slight differences in hydrophobicity.

  • For separation of intact proteins >10,000 MW
  • Great for hydrophilic proteins
  • Direct scale-up to preparative and bulk materials
  • 300 Å also available in C4 and C5 bonded phases
PhaseParticle Sizes (µm)Pore Size (Å)Surface Area (sq. m/g)Carbon Load (%)Recommended Use
C183, 5, 10, 1530017013.34Separation of hydrophilic proteins > 10,000 Da
C45, 10, 153001705Separation of highly hydrophobic proteins > 10,000 Da
C55, 103001705.5Separation of highly hydrophobic proteins > 10,000 Da; Slightly different selectivity than C4
Proteo4, 109047515Purification of synthetic peptide mixtures; Characterization of proteins by peptide mapping
Document Type:
Document Type Title
Brochures Axia- The Ultimate Pre-packed Preparative Column Add to My Documents
Other Bulk Media Selection Guide Add to My Documents
Brochures Capillary and Trap Column Solutions for Microflow LC Add to My Documents
Other Chromatography Bulk Media and PREP Guide Add to My Documents
MSDS Jupiter 10 μm C18 Add to My Documents
MSDS Jupiter 10 μm C4 Add to My Documents
MSDS Jupiter 15 μm C18 Add to My Documents
MSDS Jupiter 15 μm C4 Add to My Documents
Supporting Docs Jupiter Column Care Note Add to My Documents
Technical Notes Interferon-α Protein Modifications by HPLC (TN-1053) Add to My Documents
Other Pharmaceutical Analysis Guide- Formulations Add to My Documents
Brochures Reversed Phase HPLC Solutions for Proteins and Peptides Add to My Documents
Brochures SecurityGuard Column Protection Family Brochure (UHPLC, HPLC, PREP, SFC) Add to My Documents
Supporting Docs Troubleshooting for HPLC: Recognizing and Anticipating Challenges during Reversed Phase HPLC Add to My Documents
Technical Notes PEGylated Proteins by Reversed Phase HPLC (TN-1046) Add to My Documents
MSDS Safety Data Sheet for Silica-Based HPLC Columns Add to My Documents
Other USP Allowable Adjustments Guide Add to My Documents
Other USP Allowable Adjustments Guide Japanese (米国薬局方(USP)メソッドの許容調整) Add to My Documents
Technical Notes PEGylated Proteins by HPLC-UV (TN-1034) Add to My Documents
Supporting Docs Meeting All Your PREP Needs Add to My Documents
Other Protein and Peptide Users Guide Add to My Documents
Supporting Docs HPLC Column Care Guide Add to My Documents
Phase: Particle Size: Pore Size: Length: Internal Diameter:
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Part Number:00F-4263-B0

Description:Jupiter® 3 µm C18 300 Å, LC Column 150 x 2 mm, Ea

Required Holder:

Stationary Phase:C18

Solid Support:Fully Porous Silica

Format:Column

Separation Mode:Reversed Phase

Recommended Use:Separation of hydrophilic proteins > 10,000 Da

USP Designation:L1


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