Clarity HPLC Information

Optimized Oligo Purification and Analysis

RPC, HPLC, prep LC, desalting, and extraction solutions
DNA, RNA/RNAi, longmers, dye-labeled, and modified oligonucleotides
High efficiency LC-MS protocols for characterization/QC
Personalized technical support and customer service

Each product in the Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.

Clarity BioSolutions Product Selection

Purification

	Clarity QSP™	Clarity Oligo-RP™ Clarity Oligo-XT	Clarity Oligo-SAX™	Clarity RP-Desalting™
Primary Use	High-throughput, trityl-on RPC purification	RP-HPLC purification of failure sequences from target sequences	Economical, high loading capacity IEX-LC prep-scale purification	Quick removal or salt & excess reagent
Purities	>90%	>90%	>90%	~70%
Recoveries	~90%	~70%	>90%	~70%
Synthesis Scale Load	Up to 50 µmol	Up to 50 µmol	Up to 50 µmol	Up to 1 µmol
Oligo Types	DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Characterization / Analysis

	Clarity Oligo-RP™	Clarity Oligo-MS™ Clarity Oligo-XT	Clarity Oligo-OTX™
Primary Use	RP-LC-MS analysis with optimized selectivity and sensitivity	Rapid, high efficiency RP-LC-MS analysis for QC and characterization	Extraction of oligo therapeutics from biological samples for LC-MS bioanalysis
Oligo Length	≤ 60 mer	≤ 60 mer	≤ 40 mer
Recommended Mobile Phase	TEA / HFIP	TEA/HFIP/MeOH	N/A

Material Characteristics

Clarity Products	Particle Support	Bonded Phase	Particle shape/Size (µm)	Pore Size (Å)	Surface Area (m²/g)
Clarity QSP™ Cartridges	Polymer (PSDVB)	Hydrophilic polymer coating	35	500	300
Clarity Oligo-RP™ LC Columns	TWIN (silica, organic, composite)	C18	3, 5, 10	110	375
Clarity Oligo-WAX™ LC Columns	Silica	Crosslinked polyamine (WAX)	10	360	−
Clarity RP-Desalting™ Tubes	Silica	C18	55	140	300
Clarity Oligo-MS™ LC Columns	Core-Shell	C18	1.7, 2.6, 5	100	200* (*effective)
Clarity OTX™ Extraction Plates	Polymer (surface modified PSDVB)	Mixed-mode anion exchanger	33	85	800
Clarity Oligo-SAX LC Columns	Polymer (surface modified PSDVB)	Hydrophilic quaternary amine	5	−	−
Clarity Oligo-XT LC Columns	Core-Shell	C18	1.7, 2.6, 5	100	200

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<div id="clarityhplcpage">
<h3 style="color: #000">Optimized Oligo Purification and Analysis</h3>

<ul>
	<li>RPC, HPLC, prep LC, desalting, and extraction solutions</li>
	<li>DNA, RNA/RNAi, longmers, dye-labeled, and modified oligonucleotides</li>
	<li>High efficiency LC-MS protocols for characterization/QC</li>
	<li>Personalized technical support and customer service</li>
</ul>

<p>Each product in the Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.</p>

<div class="mrow">
<h3>Clarity BioSolutions Product Selection</h3>

<div class="mrow">
<h4>Purification</h4>

<table class="clarity-purification">
	<tbody>
		<tr>
			<th> </th>
			<th>Clarity QSP™</th>
			<th>Clarity Oligo-RP™<br />
			Clarity Oligo-XT</th>
			<th>Clarity Oligo-SAX™</th>
			<th>Clarity RP-Desalting™</th>
		</tr>
		<tr>
			<td style="text-align: left">Primary Use</td>
			<td>High-throughput, trityl-on RPC<br />
			purification</td>
			<td>RP-HPLC purification of failure<br />
			sequences from target sequences</td>
			<td>Economical, high loading capacity<br />
			IEX-LC prep-scale purification</td>
			<td>Quick removal or salt & excess reagent</td>
		</tr>
		<tr>
			<td style="text-align: left">Purities</td>
			<td>>90%</td>
			<td>>90%</td>
			<td>>90%</td>
			<td>~70%</td>
		</tr>
		<tr>
			<td style="text-align: left">Recoveries</td>
			<td>~90%</td>
			<td>~70%</td>
			<td>>90%</td>
			<td>~70%</td>
		</tr>
		<tr>
			<td style="text-align: left">Synthesis Scale Load</td>
			<td>Up to 50 µmol</td>
			<td>Up to 50 µmol</td>
			<td>Up to 50 µmol</td>
			<td>Up to 1 µmol</td>
		</tr>
		<tr>
			<td style="text-align: left">Oligo Types</td>
			<td colspan="4">DNA, RNA/RNAi, Thioates, Dye-labled, Modified</td>
			
		</tr>
		
	</tbody>
</table>

<h4>Characterization / Analysis</h4>

<table class="clarity-charac">
	<tbody>
		<tr>
			<th> </th>
			<th>Clarity Oligo-RP™</th>
			<th>Clarity Oligo-MS™<br />
			Clarity Oligo-XT</th>
			<th>Clarity Oligo-OTX™</th>
		</tr>
		<tr>
			<td style="text-align: left">Primary Use</td>
			<td>RP-LC-MS analysis with optimized<br />
			selectivity and sensitivity</td>
			<td>Rapid, high efficiency RP-LC-MS<br />
			analysis for QC and characterization</td>
			<td>Extraction of oligo therapeutics from<br />
			biological samples for LC-MS bioanalysis</td>
		</tr>
		<tr>
			<td style="text-align: left">Oligo Length</td>
			<td>≤ 60 mer</td>
			<td>≤ 60 mer</td>
			<td>≤ 40 mer</td>
		</tr>
		<tr>
			<td style="text-align: left">Recommended Mobile Phase</td>
			<td>TEA / HFIP</td>
			<td>TEA/HFIP/MeOH</td>
			<td>N/A</td>
		</tr>
		
	</tbody>
</table>

<h4>Material Characteristics</h4>

<table class="clarity-material">
	<tbody>
		<tr>
			<th>Clarity Products</th>
			<th>Particle<br />
			Support</th>
			<th>Bonded<br />
			Phase</th>
			<th>Particle<br />
			shape/Size<br />
			(µm)</th>
			<th>Pore Size<br />
			(Å)</th>
			<th>Surface<br />
			Area<br />
			(m<sup>2</sup>/g)</th>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/SPDetail/Clarity%20(SPE)/QSP?phaseTab=true" target="_blank">Clarity QSP™ Cartridges</a></td>
			<td>Polymer (PSDVB)</td>
			<td>Hydrophilic polymer coating</td>
			<td>35</td>
			<td>500</td>
			<td>300</td>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/HPLCDetail/Clarity/Oligo-RP" target="_blank">Clarity Oligo-RP™<br />
			LC Columns</a></td>
			<td>TWIN<br />
			(silica, organic, composite)</td>
			<td>C18</td>
			<td>3, 5, 10</td>
			<td>110</td>
			<td>375</td>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/HPLCDetail/Clarity/Oligo-WAX?phaseTab=true">Clarity Oligo-WAX™<br />
			LC Columns</a></td>
			<td>Silica</td>
			<td>Crosslinked polyamine (WAX)</td>
			<td>10</td>
			<td>360</td>
			<td>−</td>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/SPDetail/Clarity%20(SPE)/RP%20Desalting?phaseTab=true" target="_blank">Clarity RP-Desalting™ Tubes</a></td>
			<td>Silica</td>
			<td>C18</td>
			<td>55</td>
			<td>140</td>
			<td>300</td>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/HPLCDetail/Clarity/Oligo-MS?phaseTab=true" target="_blank">Clarity Oligo-MS™<br />
			LC Columns</a></td>
			<td>Core-Shell</td>
			<td>C18</td>
			<td>1.7, 2.6, 5</td>
			<td>100</td>
			<td>200*<br />
			(*effective)</td>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/SPDetail/Clarity%20(SPE)/OTX?phaseTab=true" target="_blank">Clarity OTX™<br />
			Extraction Plates</a></td>
			<td>Polymer (surface<br />
			modified PSDVB)</td>
			<td>Mixed-mode anion exchanger</td>
			<td>33</td>
			<td>85</td>
			<td>800</td>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/HPLCDetail/Clarity/Oligo-SAX?phaseTab=true">Clarity Oligo-SAX<br />
			LC Columns</a></td>
			<td>Polymer (surface<br />
			modified PSDVB)</td>
			<td>Hydrophilic quaternary amine</td>
			<td>5</td>
			<td>−</td>
			<td>−</td>
		</tr>
		<tr>
			<td style="text-align: left"><a href="https://www.phenomenex.com/Products/HPLCDetail/Clarity/Oligo-XT?phaseTab=true" target="_blank">Clarity Oligo-XT<br />
			LC Columns</a></td>
			<td>Core-Shell</td>
			<td>C18</td>
			<td>1.7, 2.6, 5</td>
			<td>100</td>
			<td>200</td>
		</tr>
		
	</tbody>
</table>
</div>
</div>
</div>

3 Phases

Reversed Phase 3

Featured

TECHNICAL NOTE

Analysis of Synthetic Oligonucleotides using Clarity Oligo-SAX - Quaternary Amine Functionalized, Non-porous Particles

Technical note describing characterization of various synthetic OGNs using the new Clarity Oligo-SAX.
TECHNICAL NOTE

Optimizing Sensitivity and Selectivity for the Analysis and Characterization of Synthetic Oligonucleotides via LC/MS

This technical note describes how to achieve the best sensitivity and selectivity for synthetic oligonucleotide analysis via LC/MS.
TECHNICAL NOTE

Optimization of Mobile Phase Composition for the Analysis of Synthetic Oligonucleotides using a Clarity Oligo-XT Column

This technical note describes how to best use new Clarity Oligo-XT columns for high performance analysis of synthetic oligonucleotides.
BROCHURE

Advance Your Oligonucleotide Work

See how Clarity Biosolutions can improve your sample preparation, analysis, and purification of oligonucleotides.

Oligo-RP

Particle Sizes (µm)	3, 5, 10
Pore Size (Å)	110

Compare All Phases

Oligo-RP Phase Information

Analytical LC and Purification Solutions for Oligonucleotide

Clarity Oligo-RP columns have been specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 μm particles, Clarity Oligo-RP allows for high capacity analysis and purification of oligos. Clarity Oligo-RP columns also allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.

A Highly Selective Analysis

Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA oligonucleotide due to its excellent efficiency and resolving power.

Easily Scale from HPLC to Preparative Purification

}
	
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<div id="clarityoligo-rp">
<h3>Analytical LC and Purification Solutions for Oligonucleotide</h3>

<p>Clarity Oligo-RP columns have been specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 μm particles, Clarity Oligo-RP allows for high capacity analysis and purification of oligos. Clarity Oligo-RP columns also allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.</p>

<div class="rprow" style="padding-top: 27px">
<h4>A Highly Selective Analysis</h4>
<a href="https://www.phenomenex.com/Application/Detail/16021" target="_blank"><img src="https://www.phenomenex.com/webimage/19clarity_oligo-rp_app16021.png" /></a>

<div>
<p class="app16021">Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA oligonucleotide due to its excellent efficiency and resolving power.</p>
</div>
</div>

<div class="rprow" style="padding-top: 46px">
<h3 style="color: #22bcb9;margin-bottom: 1px">Easily Scale from HPLC to Preparative Purification</h3>
<img src="https://www.phenomenex.com/webimage/19clarity_oligo-rp_dna.png" /></div>
</div>

3 Phases

Reversed Phase 3

Technical Documents
Technical Specifications
Search FAQ

Phase	Particle Sizes (µm)	Pore Size (Å)	Recommended Use
Oligo-MS	1.7, 2.6	100	Rapid LC/MS analysis and QC of synthetic oligonucleotide research reagents and therapeutics
Oligo-RP	3, 5, 10	110	Purification of synthetic oligonucleotide mixtures
Oligo-XT	1.7, 2.6, 5	100

Document Type:

Document Type	Title
Technical Notes	An Optimized Protocol for the 2 mg/well Clarity OTX Microelution 96-Well Plates	Add to My Documents
Technical Notes	An Optimized Protocol for the 25 mg/well Clarity OTX 96-Well Plates	Add to My Documents
Technical Notes	Automated Method Development of Oligonucleotide in Tissues Using Clarity OTX 96-Well Plate and High Resolution Mass Spectrometry (TN-0097)	Add to My Documents
Brochures	Axia- The Ultimate Pre-packed Preparative Column	Add to My Documents
Other	Cannabis Testing Guide 2022 (LC/GC/SP)	Add to My Documents
Other	Clarity Column Care and Use Guide	Add to My Documents
Brochures	Clarity Biosolutions for Synthetic DNA/RNA	Add to My Documents
Brochures	Clarity OTX Application Guide	Add to My Documents
Supporting Docs	Clarity Overview Flyer	Add to My Documents
Technical Notes	Extraction of 2’-MOE Phosphorothioate from Plasma Using Clarity OTX	Add to My Documents
Technical Notes	Extraction of 2’-MOE Phosphorothioate from Tissue Using Clarity OTX Solid Phase Extraction (SPE)	Add to My Documents
Brochures	Improve Your Bioanalytical Assays Today	Add to My Documents
Other	Ultimate Guide to Reversed Phase HPLC/UHPLC - Column Selectivity Selection Poster	Add to My Documents
Technical Notes	Avoiding Depurination During Trityl-on Purification (TN-0008)	Add to My Documents
Supporting Docs	FAQs for the Isolation and Purification of Synthetic Oligonucleotides	Add to My Documents
Technical Notes	Oligonucleotide Therapeutics from Biological Matrices (TN-0048)	Add to My Documents
Technical Notes	Optimization of Mobile Phase Composition for the Analysis of Synthetic Oligonucleotides using a Clarity Oligo-XT Column (TN-1200)	Add to My Documents
Technical Notes	Synthetic Oligonucleotides by LC/MS (TN-1052)	Add to My Documents
MSDS	Safety Data Sheet for Silica-Based HPLC Columns	Add to My Documents
Other	USP Allowable Adjustments Guide	Add to My Documents
Other	USP Allowable Adjustments Guide Japanese (米国薬局方（USP)メソッドの許容調整)	Add to My Documents
Supporting Docs	Meeting All Your PREP Needs	Add to My Documents
Other	Protein and Peptide Users Guide	Add to My Documents
Brochures	Clarity Biosolutions for Synthetic DNA/RNA - Japanese	Add to My Documents
Brochures	Clarity OTX- Japanese	Add to My Documents
Supporting Docs	HPLC Column Care Guide	Add to My Documents

Phase: Particle Size: Pore Size: Length: Internal Diameter:

Part Number:00F-4445-V0-AX

Description:Clarity® 10 µm Oligo-RP, LC Column 150 x 50 mm, AXIA™ Packed, Ea

Required Holder:

Stationary Phase:C18

Solid Support:Fully Porous Organo-silica

Format:AXIAL Compression

Separation Mode:Reversed Phase

Recommended Use:Purification of synthetic oligonucleotide mixtures

USP Designation:L1

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Oligonucleotide

Purification Products