A Rapid, Automatable Solution for Phosphatidylethanol (PEth) LC-MS/MS Analysis in Whole Blood

Abstract:

Phosphatidylethanol (PEth) has emerged as an ethanol specific marker for alcohol consumption. PEths are phospholipids with very similar structures, which makes them challenging to separate chromatographically. In this fast analysis, a Kinetex® Core-Shell Biphenyl LC column was used with a direct, in-plate phospholipid enrichment and cleanup for PEth from whole blood. The automatable LC-MS/MS workflow, including sample preparation and column selection, greatly simplifies the analysis while achieving excellent analytical performance.

Document Type:

Technical Notes

Industries:

Clinical

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Kinetex Phree

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