A Selective Method for Quantitation of Underivatized Methylmalonic Acid (MMA) in Plasma
- Abstract:
- In this technical note, we present a fast, reproducible LC/MS/MS method to analyze underivatized MMA by utilizing a unique Luna® Omega 1.6 μm PS C18. The method runtime is 5 minutes including column re-equilibration. For sample preparation procedure we used Strata®-X-AW solid phase extraction to produce a clean sample from plasma. Analyte detection was performed using negative mode electrospray ionization of a triple quadrupole MS.
- Document Type:
- Technical Notes
- Industries:
- Clinical
- Techniques:
- HPLC / UHPLC
- Brands:
- Luna Strata-X
- PDF Link:
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