A Sensitive Extraction and Analysis of Urinary Catecholamines Using Strata®-X-CW Microelution Solid Phase Extraction (SPE) and Kinetex® Biphenyl HPLC Column (TN-0106)

Abstract:

Metanephrine and normetanephrine are both metabolites of epinephrine and norepinephrine. In this technical note we will explore how to use Strata-X-CW Microelution SPE 96-Well Plates in conjunction with a Kinetex Biphenyl HPLC column in order to resolve an interference that coelutes with 3-Methoxytyramine on a standard C18 HPLC column, while reaching low limits of quantification for specific urinary catecholamines, metanephrine and normetanephrine.

Document Type:

Technical Notes

Industries:

Clinical

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Kinetex Strata-X

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