A Simple Approach to Method Development using Analyte LogD TN-1236

Abstract:

In this example, an ibuprofen standard was obtained and screened on 3 different reversed phase Kinetex core-shell columns at two separate pH’s, 2.0 and 8.0. The values of mobile phase pH were selected to correspond to ibuprofen’s pKa of 4.9 and identified by referencing Figure 2. The purpose of the selection of two distinct pH values was to demonstrate the solubility implications of the fractional composition of the ionized vs. unionized species of the drug. Furthermore, we demonstrated how LogD is a useful and fast indicator in determining the most appropriate mobile phase pH for a given method.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Kinetex

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