Comparison of two particle morphologies and four C18 phases when applied to the underivatized retention of five carboxylic acids under typical

Abstract:

The goal of this application was to demonstrate a simple yet effective separation of five common organic acids under typical reversed phase conditions amenable to all laboratory situations. Polar stationary phase modifications, as well as embedded charged functionalities, were also included in this study. The effect of particle morphology on the separation of five organic acids were within the scope of this investigation (Table 1). For this study, we evaluated Kinetex® core-shell (superficially porous) and Luna® Omega fully porous particles and looked at the same stationary phase chemistries bonded to each. After selecting the most relevant stationary phase and particle size, flow rate and column length were adjusted to optimize chromatographic effect and performance.

Document Type:

Technical Notes

Industries:

Food & Beverage

Techniques:

HPLC / UHPLC

Brands:

Luna

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