Determination of Sugars in Animal Feed using HPLC-ELSD (TN-1234)

Abstract:

In this work, a HPLC-ELSD method was evaluated for the analysis of six free nutritional sugars in animal feed: fructose, galactose, glucose, sucrose, maltose, and lactose. Assay performance including precision and recovery were evaluated and met the acceptance criteria. A Luna® Omega SUGAR LC Column was selected for its ability to retain polar sugar molecules and differentiate individual sugars using HILIC conditions. The HPLC methodology allowed for a simple sample extraction with ethanol and water followed by filtration.

Document Type:

Technical Notes

Industries:

Food & Beverage

Techniques:

HPLC / UHPLC

Brands:

Luna

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