Enantiomeric Analysis of Dextromethorphan & Levomethorpan and Dextrorphan & Levorphanol in Urine and Serum by LC-MS/MS (TN-1267)

Abstract:

Dextromethorphan is a drug found in over the counter cough medicine, while its stereoisomer levomethorphan is an opioid and Schedule II Narcotic that has not been marketed. Dextromethorphan is metabolized to dextrorphan, and levomethorphan is metabolized to levorphanol. Levorphanol, stereoisomer of dextrorphan, is also available by prescription for pain management. These sets of enantiomers are indistinguishable by mass spectrometry and not resolved by typical reversed phase chromatography methods. The LC-MS/MS method presented here uses chiral chromatography to identify and quantitate the individual enantiomers present in both urine and serum.

Document Type:

Technical Notes

Industries:

Clinical Forensics/Toxicology

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Lux Strata-X

PDF Link:

Click here