Enhancing Sensitivities and Peak Capacities for UHPLC-MS Fast Gradient Analyses (TN-1104)

Abstract:

When compared to 1.7 μm fully porous materials, the ultra-high efficiency and low backpressures provided by Kinetex core-shell 2.6 μm columns, provides users opportunities to go beyond what is traditionally accepted for UHPLC runs. To match backpressures exerted by 1.7 μm fully porous columns, large increases in core- shell column length and/or system flow rate is required. When gra - dient conditions are maintained, increases in flow rate and length result in an increase in peak capacity on the order of 26 %. When MS conditions are optimized to compensate for new flow rates, sensitivity increases of up to 3.5 times are observed and further more, peak capacity figures increase by almost 70 %.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Kinetex

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