Enhancing Sensitivity and Peak Capacity for Protein Digest using Micro-LC and the power of 2.6 μm Kinetex Core-Shell Columns (TN-1285)

Abstract:

In this technote we compare the performance of the Kinetex 2.6 μm core-shell packed into 0.3 mm ID columns to a market leading 1.7 μm fully porous UHPLC alternative and demonstrate the average peak widths and peak capacities seen between a 2.6 μm core-shell material are comparable to a sub 2 μm fully porous whilst operating at a significantly lower back pressure. Furthermore, the reduction in back pressure can facilitate the use of longer columns, something which has the potential to be extremely beneficial when the goal is maximizing peak capacity.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Kinetex

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