Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life

Abstract:

In this technical note, we evaluate the hydrolysis efficiency of IMCSzyme RT by 1) hydrolyzing 4 human urine samples at a range of enzyme amounts, and 2) hydrolyzing 96 human urine samples at a fixed amount of enzyme. We also evaluated how sample treatment using β-Gone™ Plus β-glucuronidase removal plates can improve column life and peak quality of analytes compared to Dilute-and-Shoot treatment methods, using a Kinetex™ 2.6 µm Biphenyl LC column.

Document Type:

Technical Notes

Industries:

Clinical Forensics/Toxicology

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Beta-Gone Kinetex

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