Low Molecular Weight Separation by GFC (TN-1083)

Abstract:

To obtain the optimal resolution of low molecular weight peptides and proteins by Gel Filtration Chromatography (GFC), one must use unique mobile phase conditions compared to the near-physiological conditions used for protein separation. For peptides, organic solvents and ion-pairing reagent (TFA) are used to minimize interactions resulting in sharp peaks with distinct retention typically based on the molecular weight of the peptide on a BioSep-SEC-S2000 GFC column

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

BioSep-SEC-S

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