Improving Resolution for Size Exclusion Chromatography Methods by Optimization of Linear Velocity

Abstract:

Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is a unique separation modality in that lower flow rates often yield improvements in separation and resolution can improve quite considerably simply by running at a lower linear velocity. This application note demonstrates the utility of method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb, a common standard protein.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

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