Investigating the effect of column ID on sensitivity when using Micro LC columns

Abstract:

In this application note, we investigate the ability to scale separations developed on traditional analytical columns (2.1 mm ID) to micro flow rate scale columns (0.3 and 0.5 mm ID) to take advantage of the sensitivity gains through miniaturization without the need for new method development and optimization. The comparison was generated using a representative sample of 20 stable-isotope-labelled (SIL) peptides under typical reversed-phase mobile phase conditions using a SCIEX® 5500 QTRAP MS/MS detector. This application demonstrates the effect on sensitivity and selectivity when column ID is reduced into a micro LC format.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Kinetex Luna

PDF Link:

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