Investigating the Performance Attributes of Stationary Phase Selectivity and Particle Morphology in Nano LC-MS

Abstract:

The miniaturization of LC columns is a powerful tool to increase MS sensitivity. By combining nano LC column technology with highly efficient column packing materials such as core-shell and thermally modified particles can increase the benefits through narrower chromatographic peaks. Narrower peaks lead to higher sensitivity as well as increases in the number of protein and peptide identifications by as much as 10%. As with analytical scale LC separations, the choice of column chemistry can greatly affect the separation. Having multiple selectivity options in nano LC columns allows one to tailor the chromatography for the compounds of interest to the researcher.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

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