PEGylated Proteins by HPLC-UV (TN-1034)

Abstract:

Reversed phase separations were performed on PEGylated and non-PEGylated forms of proteins in an attempt to better understand the various parameters that affect separations. Different proteins were evaluated, as were peptides, in an effort to derive more universal separations of PEGylated proteins from native proteins. HPLC column chemistries were evaluated as well as mobile phase organic composition, temperature, gradient conditions, and pH. Unlike gel filtration, reversed phase separations demonstrate excellent selectivity for proteins based on the specific site of PEGylation.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Jupiter

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