Metformin from Human Plasma by Phospholipid Removal and LC-MS/MS (TN-1243)

Abstract:

The fast analysis time demonstrated for the assay of metformin in human plasma would be ideally suited for a bio-equivalence study. Here we have demonstrated an LC-MS/MS assay with a total run time of 2 minutes using a Kinetex C18 5 μm, 50 x 4.6 mm column. Sample preparation with the Phree 96-well plate is an automation friendly format, which gave very clean extraction results by minimizing the possible matrix interference peaks from endogenous phospholipids and proteins.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Kinetex Phree

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