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Optimizing GPC (TN-1100)


Abstract:
Gel Permeation Chromatography (GPC) is principally used to separate polymers and organic molecules based on their size. An important aspect in optimizing GPC separations is column selection, especially when multiple columns are used. Mobile phase selection and column temperature are also important factors that must be considered when developing a method, as they can influence the solubility and viscosity of diluent and analyte respectively. Column, mobile phase, and temperature selection will be discussed to optimize GPC methods using Phenogel GPC/SEC columns.
Document Type:
Technical Notes
Industries:
Pharmaceutical/Biopharmaceutical Environmental Food & Beverage Fuels Personal Care/Consumer Products Agriculture Specialty Chemical/Industrial
Techniques:
HPLC / UHPLC
Brands:
Phenogel
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