Peptide Calibration Mix using Kinetex 2.6 µm XB-C18 Core-Shell Column Compared with 1.7 µm Fully Porous Column

Abstract:

The Kinetex 2.6 µm XB-C18 micro-UHPLC columns provide exceptional peak efficiency with which to resolve and evaluate the many peaks of a complex peptide mixture without generating the corresponding backpressure. The superficially-porous nature of the Kinetex “core-shell” particles feature shorter pore pathways that mimic the efficiency of smaller fully-porous particles, while also mitigating the backpressure associated with UHPLC efficiency. In this application note we show that a peptide calibration standard demonstrates the higher peak capacity, higher efficiency, and lower backpressure of the Kinetex 2.6 µm XB-C18 media when compared with competing fully-porous micro-UHPLC columns that share the same hardware dimensions.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Kinetex

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