Peptide Quantitation with a bioZen Peptide PS C18 Column Following Antibody Enrichment Through Ligand Binding with bioZen MagBeads (TN-0136)

Abstract:

In this application, we present a LC-MS method for peptide quantitation which uses bioZen MagBeads Streptavidin coated paramagnetic beads to capture and isolate biomolecules through a LBA. In this specific technical note, we look at the recovery of signature peptides from Rituximab comparing bioZen Magbeads and other Streptavidin magnetic beads products for batch-to-batch reproducibility. Due to the properties of the LC-MS analysis, reproducibility of lot-to-lot recovery is significant when looking at robust peptide quantitation methods.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC Sample Prep

Brands:

bioZen (SPE) bioZen

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