Quantitation of Clinical Research Pain Panel Analytes from Oral Fluid Utilizing Microelution Solid Phase Extraction Coupled with LC-MS/MS Poster

Abstract:

Oral fluid has emerged as a popular biological matrix for analysis due to its non-invasive nature and ease of sample collection. It has wide applicability for drug testing and screening in clinical research. However, the analysis of these compounds in oral fluid becomes challenging due to the presence of the excipients, surfactants, and preservatives in the collection buffer of commercially available oral fluid collection (OFC) devices. These additives are necessary to ensure the stability and authenticity of the sample during collection and transport. However, the presence of these additives can foul the optics of the mass spectrometer and diminish the signal response if the samples are not cleaned up adequately before injection. In this communication we present an effective sample cleanup method for oral fluid analysis that targets 32 pain panel analytes, utilizing a mixed mode strong cation exchange Strata™-X-C microelution 96-well plate. A Kinetex™ core-shell 2.6 µm Biphenyl, 50 x 4.6 mm column was employed for fast chromatographic separation

Document Type:

Poster

Industries:

Clinical Pharmaceutical/Biopharmaceutical Forensics/Toxicology

Techniques:

Sample Prep

Brands:

Strata-X Kinetex

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