Selectivity Differences in Size-Exclusion Chromatography for Monoclonal Antibodies; How Pore Size Effects Retention (TN-1251)

Abstract:

The scope of this study is to demonstrate the selectivity differences that can be observed when working with column packing media of different pore sizes. The bioZen SEC-2 media has a 150Å pore and is typically recommended for the separation of smaller mAbs and antibody fragments. The bioZen SEC-3 contains a 300Å pore size that is better for separation of mAb aggregates from monomer. This technote will present the results from this study using two well-characterized mAbs, rituximab and trastuzumab.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

PDF Link:

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