Separation of an Amino Acid Mixture in HILIC Mode Using a Luna ™NH 2 Column

Abstract:

In this application note, a Luna 3 µm NH 2 column, in HILIC mode, and a robust LC MS/MS method was used to successfully separate an amino acid mixture. Amino groups bound to the silica surface of the stationary phase particles serve as a weak anion exchanger and offer greater polar selectivity, thus increased separation of highly polar amino acids.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

Amino Acids HPLC / UHPLC

Brands:

Luna

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