Simultaneous Detection of Tricarboxylic Acid Cycle Intermediates using LC-MS/MS with a Synergi™ Fusion-RP HPLC Column

Abstract:

In this technical note, the goal was to develop an LC-MS/MS assay able to analyze tricarboxylic acids out of a human serum albumin matrix over a dynamic range of concentrations and demonstrate acceptable accuracy and precision in reference to GLP guidance. In addition, the reference method should be a fast and reproducible assay, easily implemented in most laboratories with standard analytical equipment.

Document Type:

Technical Notes

Industries:

Clinical Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

Synergi

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