Size Exclusion Chromatography for the Determination of Protein Hydrodynamic Radius - Revisiting the Calibration Curve

Abstract:

Size exclusion chromatography (SEC) is a non-adsorptive chromatographic modality where large molecules, such as proteins or polymers separate based upon hydrodynamic radius. Larger molecules which cannot permeate all the pores in the chromatographic media are partially excluded, thus elute earlier, wherein smaller molecules which can permeate more pores, elute later. An understanding of analyte hydrodynamic radius (Rh), as well as size exclusion column pore size and distribution, are all critical in the understanding of how an analyte will behave by SEC. In this white paper, we will explore several protein standards with known hydrodynamic radii, and how this can be used to determine ideal size exclusion conditions for optimal method performance

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

PDF Link:

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