The Effect of Column Internal Diameter for Robust Size Exclusion Chromatography Methods

Abstract:

The use of sub-2 µm particles for SEC have expanded analytical method for the characterization of biologics. This is especially noteworthy with monoclonal antibodies (mAbs), wherein methods developed on ultra high-performance SEC (UHP-SEC) columns can separate not only aggregates, but also fragment. This application note explores the effect of column internal diameter for robust size exclusion chromatography methods.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

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