mRNA Poly A Tail Degradation Detection on a Biozen Oligo Column

Abstract:

Understanding the degradation of the poly A tail is an essential tool to improving delivery of therapeutics. In this application note, mRNA oligonucleotides strands of 1250 nucleotides were enzymatically digested using RNase H followed by injection into a Biozen 2.6 μm Oligo column. We demonstrate the ability to distinguish a naturally degraded poly A tail with single nucleotide resolution through Liquid Chromatography (LC) and High-Resolution Mass Spectrometry (HRMS).

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

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