Use of Verex Filter Vials for Monoclonal Antibody (mAb) Fragments

Abstract:

In this application, an IgG1 (Trastuzumab) and an IgG4 (Nivolumab) are digested with IdeZ protease, yielding Fab2’ and scFc fragments. Digested sample was then filtered using Verex Filter Vials, with either Regenerated Cellulose (RC) or Polyethersulfone (PES) membranes. Samples were then run by RPLC to determine if there was a loss of sample.

Document Type:

Technical Notes

Industries:

Cannabis Pharmaceutical/Biopharmaceutical Clinical Environmental Food & Beverage Forensics/Toxicology Fuels Personal Care/Consumer Products Agriculture Specialty Chemical/Industrial Life Science

Techniques:

Accessories HPLC / UHPLC Sample Prep

Brands:

VerexF

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