Utilizing Biphasic Salt and pH Gradients for Charge Variant Analysis (TN-1252)

Abstract:

Cation exchange chromatography (CEX) is a favorable analytical method for characterization of proteins as it is non-denaturing and can be used to qualitatively and quantitively identify charge heterogeneity of a protein samples. This technote will describe mobile phase effects on weak cation exchange (WCX) chromatography including a biphasic salt and pH gradient.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Brands:

bioZen

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