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Morphine and Metabolites on Luna 5um HILIC

Application Detail (App ID: 16334)

Part No:00D-4450-B0
Dimensions:100 x 2 mm ID
Elution Type:Gradient
Elution A:90/10 Acetonitrile/100mM Ammonium Formate pH 3.2
Elution B:50/50 Acetonitrile/20mM Ammonium Formate pH 3.2
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 1 100 0
3 3 0 100
4 4.5 0 100
5 4.51 100 0
6 9.51 100 0

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ABI 3000 Triple Quad MS
Note:The pH of the aqueous buffer is specified. The pH in mixed organic was not measured.

Analyte Details

Analyte Detail not available

1
Morphine
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2
Normorphine
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3
Morphine-3-beta-D-glucuronide
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