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Nicotine and Metabolites on Luna 5um HILIC

Application Detail (App ID: 16335)

Part No:00D-4450-B0
Dimensions:100 x 2 mm ID
Elution Type:Isocratic
Elution A:90/10 Acetonitrile/100mM Ammonium Formate pH 3.2
Gradient Profile:
Step No. Time (min) Pct A
1 0 100

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ABI 3000 Triple Quad MS
MRM transitions monitored:
Nicotine: m/z 163.3/105.9 and 163.3/80.1
Nornicotine: m/z 149.1/80.1
Cotinine: m/z 177.3/98.0 and 177.3/80.1
Note:The pH of the aqueous buffer is specified. The pH in mixed organic was not measured.

Analyte Details

Analyte Detail not available

1
Cotinine
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2
Nicotine
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3
Nornicotine
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