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Water-soluble Vitamins on Kinetex 2.6u, C18, 150x4.6 by HPLC

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Application Detail (App ID: 20000)

Column: Kinetex® 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Part No:00F-4462-E0

Dimensions:150 x 4.6 mm ID

Elution Type:Gradient

Elution A:3g Sodium 1-hexanesulfonate monohydrate/0.4mL Triethylamine/15mL Acetic acid in 1985mL H2O

Elution B:Methanol

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
2	12	53	47
3	12.1	2	98
4	15	2	98
5	15.1	95	5
6	22	95	5

Flow Rate:0.5 mL/min

Col. Temp: 25 °C

Detection: UV-Vis Abs.-Variable Wave.(UV) @ 270 nm (22°C)

Analyte Details

Analyte Detail not available

Ascorbic acid

Pyridoxal 5'-phosphate

Nicotinic acid

4-Pyridoxic acid

Nicotinamide

Riboflavin 5'-phosphate

Pyridoxal

Pyridoxine

Cobalamine

Folic acid

Pyridoxamine

Riboflavin

Thiamine