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Sulfonamides in Meat by LC/MS/MS

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Sample Preparation Details

Part Number :8B-S100-UBJ

Description :Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk

Condition 1 :4 mL Methanol

Condition 2 :4 mL 0.1% Formic Acid in Water

Load :Load 4.00 mL Sample diluted in 0.1% Formic Acid/Methanol (95:5)

Wash 1 :2 mL 0.1% Formic Acid in Water

Dry :2min at 10inches Hg

Elution 1 :1 mL Methanol

Evaporate: :Evaporate to dryness

Reconstitute :250 µL 90:10 water:methanol, containing 0.1% formic acid

Application Detail (App ID: 20036)

Column: Gemini® 3 µm C18 110 Å, LC Column 50 x 4.6 mm, Ea

Part No:00B-4439-E0

Dimensions:50 x 4.6 mm ID

Elution Type:Gradient

Elution A:0.1% Formic Acid in Water

Elution B:Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	98	2
2	2	98	2
3	2.1	85	15
4	6.5	50	50
5	7.1	0	100
6	8	0	100
7	8.1	98	2
8	10	98	2

Flow Rate:800 µL/min

Col. Temp: ambient

Detection: Tandem Mass Spec (MS-MS) @ (300°C)

Detector Info: SCIEX 3200 QTRAP®

Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Sulfadiazine

Sulfathiazole

Sulfapyridine

Sulfamerazine

Sulfamethazine

Sulfamethoxazole

Sulfadimethoxine

Sulfaquinoxaline