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EPA 539 on Gemini NX-C18 3 um 100 X 2.0 mm

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Application Detail (App ID: 21939)

Column: Gemini® 3 µm NX-C18 110 Å, LC Column 100 x 2 mm, Ea

Part No:00D-4453-B0

Dimensions:100 x 2 mm ID

Elution Type:Gradient

Elution A:0.2%NH4OH in water

Elution B:0.2%NH4OH in MeOH

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	65	35
2	0.01	65	35
3	0.6	35	65
4	7.5	35	65
5	8.5	15	85
6	13	15	85
7	13.01	65	35
8	15	65	35

Flow Rate:0.2 mL/min

Col. Temp: ambient

Detection: Tandem Mass Spec (MS-MS) @ (22°C)

Analyte Details

Analyte Detail not available

Estriol

Bisphenol A-d16

Equilin

Estrone

beta-Estradiol

Ethynylestradiol-d4

17a-Ethynlestradiol

Androstenedione

Testosterone-d3

Testosterone

IS-13C6-estradiol

IS-Estriol d2

IS-13C2-Ethynylestradiol