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Charge Variants of Cetuximab using Salt Gradient on bioZen 6 µm WCX

Application Detail (App ID: 25059)

Part No:00G-4777-E0
Dimensions:250 x 4.6 mm ID
Elution Type:Gradient
Elution A:20 mM MES, pH 5.6
Elution B:20 mM MES, 300 mM NaCl, pH 5.6
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 1 85 15
3 31 55 45
4 31.1 0 100
5 34 0 100
6 35 85 15

Flow Rate:1 mL/min
Col. Temp: 25 °C
Detection: LC/UV (DAD, PDA) @ 280.0000000000 nm (nanometers) (0°C)
Note:*Sample graciously gifted by Catherine Bladen, Absolute Antibody

Analyte Details

Analyte Detail not available

1
Cetuximab
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